Chloramphenicol ELISA test Kit
Catalog No. LSY-10007
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Incubation Temperature: 25?
Incubation Time: 30min?30min?15min
Detection limit:
Egg, milk (Method 1) 0.1 ppb
Urine, serum, intestine, feed, milk powder 0.05 ppb
Tissue, liver, honey, milk (Method 2) 0.025 ppb
Recovery rate
Tissue, liver 85±20%
Honey, intestine 83±25%
Milk, feed 75±23%
Urine, serum
70±18%
Cross-reaction rate:
Chloramphenicol 100%
Thiamphenicol < 0.1%
Florfeniol < 0.1%
3. Components
1? Micro-well strips: 12 strips with 8 removable wells each
2? 6Χ standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
3? Enzyme conjugate (12 mL) red cap
4? Concentrated antibody working solution (1 mL) blue cap
5? Substrate A solution (7 mL) white cap
6? Substrate B solution (7 mL) black cap
7? Stop solution (7 mL) yellow cap
8? 20Χ concentrated washing buffer (40 mL) white cap
9? 2Χ concentrated redissolving solution (50 mL) transparent cap
| Model/Article number: | LSY-10007 |
| Place of origin: | Shenzhen,China |
| Brand: | Green Spring |
| Price: | 120 USD |
| Price Terms: | FOBShenzhen |
| Packing: | By foam box with ice to maintain cool storage |
| Delivery time: | 3 days |
| Minimum order: | 1 kit |
| Quality/Certifications: | ISO9001:2008 Certificate |
Category: Chemicals & Plastics -> Chemical products -> Chemical preparations and reagents
Shenzhen Lvshiyuan Biotechnology Co.,Ltd |
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| Street: | No.2 Binhai Road, Dapeng | ||||||
| City: | Shenzhen | ||||||
| Postal code: | 518120 | ||||||
| Country: | China | ||||||
| Phone: | +86 755 28438788 | ||||||
| Fax: | +86 755 28938800 | ||||||
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Contact person |
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| Name and Surname: | Bella Zou | ||||||
| Phone: | +86 755 28438788 | ||||||
| Mobile: | +86 13427908554 | ||||||