Tetracyclines(TCs) ELISA Test Kit
Catalog No. LSY-10006
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tetracyclines in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tetracyclines in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tetracyclines antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tetracyclines in it. This value is compared to the standard curve and the Tetracyclines concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.05 ppb
Incubation Temperature: 37℃
Incubation Time: 30min—30min—15min
Detection limit:
Tissue (chicken, duck, pork/liver, shrimp, fish, egg):
Tetracyclines 0.3ppb
Minocycline 0.3ppb
RoliTetracyclines 0.3ppb
Aureomycin 0.3ppb
Demethylchlor Tetracyclines 0.6ppb
Terramycin 0.4ppb
Doxycycline 0.6ppb
Honey
Tetracyclines 2ppb
Minocycline 2ppb
RoliTetracyclines 2ppb
Aureomycin 2ppb
Demethylchlor Tetracyclines 6ppb
Terramycin 4ppb
Doxycycline 6ppb
Cross-reaction rate:
Tetracyclines 100%
Minocycline 125%
RoliTetracyclines 110%
Aureomycin 100%
Demethylchlor Tetracyclines 35%
Terramycin 58%
Doxycycline 45%
Recovery rate:
Tissue (chicken, duck, pork/liver, shrimp, fish) 85±20%
Honey 75±20%